Age-related changes in lysosomal abundance in mouse hearts assessed by Lysotracker fluorescence imaging and autophagy gene expression analysis.

Jawaher Albulushi, Hannah Coghlan, Mohesh Moothanchery, Aiswarya Dev, Emily Akerman, Jasmine Heenan, Nordine Helassa, Oluwatobi Adegbite, Parveen Sharma, Fenil Patel, Libby Harrison, Mahon L Maguire, Gary R Mirams, Pawel Swietach, Harish Poptani, Rebecca Ab Burton

Institute of Systems, Molecular and Integrative Biology, Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK.Institute of Systems, Molecular and Integrative Biology, Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK. · pubmed

Paper

Abstract

Lysosomal function is essential for cardiac proteostasis and cellular health, yet its regulation during ageing remains poorly defined. We aimed to determine whether whole-organ, fluorescence imaging using an In Vivo Imaging System (IVIS) provides a novel, rapid and scalable approach for quantifying lysosomal abundance in intact ex vivo hearts prior to deeper molecular analysis.

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Research summary

The paper claims that fluorescence imaging can quantify lysosomal abundance in mouse hearts to better understand age-related changes. This research is relevant as it investigates the role of lysosomal function in cardiac health during aging, which is a key aspect of understanding the biological mechanisms of aging and potential interventions.

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