Polysaccharide-mediated aging-modulatory effects of Labisia Pumila extracts on the physiological changes in C. elegans and D. melanogaster.

Abstract

Polysaccharides are natural macromolecules with diverse bioactivities and potential health benefits. It exhibits a broad spectrum of biological effects, with extensive research supporting their antioxidative, anti-inflammatory, immunomodulatory, and anti-aging properties. The study evaluated the aging-modulatory effects of Labisia pumila polysaccharides (LPP) using Caenorhabditis elegans (C. elegans) and Drosophila melanogaster (D. melanogaster) as model organisms. LPP treatment increased the lifespan of in both models, enhanced locomotor activity and improved stress tolerance under the variety stress conditions. The researches using C. elegans mutant strains suggested that these effects are associated with the conserved stress response pathways DAF-16 and SOD-3, indicating potential involvement in cellular defense mechanisms. Metabolomic profiling further indicated increased levels of antioxidant metabolites and altered energy metabolism pathways, suggesting improved cellular homeostasis. These effects align with hormesis, in which mild stress induced by LPP triggers endogenous protective mechanisms to enhance physiological resilience. Overall, the results show that LPP modulates aging through integrated changes at the behavioral, genetic, and metabolic levels, offering a mechanistic foundation for its potential use as a safe and effective natural agent in promoting healthy aging.