Epigallocatechin gallate mitigates oxidative stress-induced transient senescence and injury by preserving mitochondrial integrity and restoring redox-inflammatory homeostasis in murine macrophages.

Abstract

Macrophages serve as major defenders against pathogens, playing a crucial role in the initiation and modulation of immune responses. Age-related decline in macrophage functions is attributed to a complex network of cellular senescence and immunosenescence. The onset of cellular senescence is often a consequence of sustained oxidative stress, which is worsened by immunosenescence. Green tea catechin, epigallocatechin gallate (EGCG), has emerged as a promising candidate for promoting healthy aging by reducing cellular senescence and enhancing cytoprotective responses. However, its effectiveness in preventing oxidative stress-induced senescence and dysfunction in immune cells, particularly macrophages, remains less characterized. The present study explored the anti-senescence and cytoprotective effects of EGCG in the wake of oxidative stress-induced premature senescence in RAW 264.7 macrophages. Oxidative stress was induced in cells using repeated exposure to hydrogen peroxide (H