Monitoring senescent cell dynamics in wound healing using a p16-tdtomato mouse model.

Qiaoling Wang, Maria Shvedova, Magda Abdelkader, Daniel S Roh

Division of Plastic & Reconstructive Surgery, Department of Surgery, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA.Division of Plastic & Reconstructive Surgery, Department of Surgery, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA. · pubmed

Paper

Abstract

Although accumulating evidence implicates cellular senescence in acute wound healing, the precise roles of senescent cells within distinct cell lineages during this process remain elusive. To address this, we employed the p16-tdTomato reporter mouse model for labeling and isolating senescent cells from wound tissue. Longitudinal in vivo imaging monitoring revealed the temporal dynamics of tdTomato (tdTom) fluorescence, with signal detection as early as postoperative day 3, peaking by day 6. Utilizing an optimized tissue digestion protocol, we achieved high-viability FACS isolation of p16

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Research summary

The paper investigates the dynamics of senescent cells in wound healing using a specific mouse model. This research is relevant as it explores cellular senescence, a key factor in aging, and its role in tissue repair, which could inform strategies for promoting healthier aging and longevity.

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