Identification of skeletal muscle stem cell adhesion motifs using spot-synthesis-based peptide arrays.

Elizabeth Leblanc, Svenja C Schüler, Yuguo Liu, Léa Théroux, Emmeran Le Moal, Marc-André Bonin, Pierre-Luc Boudreault, C Florian Bentzinger

Département de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche Du Centre Hospitalier Universitaire de Sherbrooke, Faculté de Médecine et des Sciences de La Santé, Université de Sherbrooke, Sherbrooke, QC, Canada.Département de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche Du Centre Hospitalier Universitaire de Sherbrooke, Faculté de Médecine et des Sciences de La Santé, Université de Sherbrooke, Sherbrooke, QC, Canada. · pubmed

Paper

Abstract

Altered interactions with the extracellular matrix (ECM) represent a root cause of skeletal muscle stem cell (MuSC) dysfunction in aging and disease, underscoring the therapeutic potential of targeting adhesion receptors. Here, we describe the development of an approach for the medium-throughput screening of bioactive ECM-derived adhesion motifs using peptide arrays generated by highly parallel SPOT synthesis. Based on a library of ∼50 peptide sequences originating from ECM proteins, we identified several candidate motifs that robustly enhance the adhesion of MuSC-derived cells. We demonstrate that these peptide motifs can improve the

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Research summary

The paper identifies bioactive ECM-derived adhesion motifs that enhance the adhesion of skeletal muscle stem cells. The research addresses the dysfunction of muscle stem cells in aging, which is a root cause of age-related decline in muscle regeneration and function.

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