Age-dependent reprogramming of vascular metabolism compromises endothelial resilience to inflammation-induced endothelial dysfunction.

Agnieszka Karaś, Elżbieta Buczek, Janusz Pyka, Isabelly Annunciato, Barbara Kutryb-Zając, Agata Jędrzejewska, Klaudia Stawarska, Anna Bar, Anna Kurpińska, Anni I Nieminen, Csaba Szabo, Patrycja Kaczara, Stefan Chłopicki

Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics, Krakow, Poland; Jagiellonian University, Doctoral School of Exact and Natural Sciences, Krakow, Poland.Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics, Krakow, Poland; Jagiellonian University, Doctoral School of Exact and Natural Sciences, Krakow, Poland. · pubmed

Paper

Abstract

Endothelial dysfunction is a hallmark of vascular inflammation and aging. However, the role of cellular bioenergetics in the age-dependent resilience of endothelial function to inflammation has not yet been characterized. Therefore, the aim of this study was to determine whether IL-1β-induced inflammation differentially influences vascular metabolism and endothelial function in the isolated aorta of aged mice compared to young mice. Aorta from young (3-8 months) and old (22-28 months) C57BL/6 mice was isolated and subjected to ex vivo analysis of endothelial function using wire myography and vascular NO production by electron paramagnetic resonance spectroscopy (EPR). Cellular bioenergetics was assessed with the Seahorse extracellular flux analyzer for the measurement of mitochondrial respiration and glycolysis.

The following analysis is generated by AI and may contain errors. It is separate from the authors’ abstract and is not peer review.

Research summary

The study claims that age-dependent changes in vascular metabolism affect endothelial resilience to inflammation. This research is relevant as it investigates the underlying mechanisms of endothelial dysfunction in aging, which is a critical aspect of age-related vascular diseases and longevity.

Author of this paper? Send a correction.