Establishment and application of a fully automated serum total free sulfhydryl assay based on 2,2'-DTDP.

Jie Zeng, Zhiyu Shao, Siming Wang, Jun Dong, Weiyan Zhou, Haijian Zhao, Tianjiao Zhang, Jiangtao Zhang, Jing Wang, Hao Zheng, Wenxiang Chen, Chuanbao Zhang

National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, 100730, China.National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, 100730, China. · pubmed

Paper

Abstract

Serum free thiols (FTs) are sensitive biomarkers of oxidative stress, closely associated with aging and chronic disease risk. Existing DTNB-based detection methods suffer from product instability, affecting analytical reliability. A more stable and high-throughput method is needed.

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Research summary

The paper claims to establish a more stable and high-throughput method for measuring serum free thiols. This is relevant as serum free thiols are biomarkers of oxidative stress, which is closely linked to aging and chronic disease risk.

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