Single-cell mapping reveals age-related alterations in periosteal progenitor cells and immune microenvironment.

Lei Zhao, Chao Wu, Keran Chen, Zhaoning Xu, Yu You, Peiru Zhao, Di Zhu, Meiling Su, Jian Luo, Yiyun Wang

Shanghai YangZhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), School of Medicine, Tongji University, Shanghai, China.Shanghai YangZhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), School of Medicine, Tongji University, Shanghai, China. · pubmed

Paper

Abstract

Aging profoundly impacts bone homeostasis and regeneration, yet the cellular and molecular mechanisms underlying periosteal aging remain poorly understood. Using single-cell RNA sequencing, we profiled the periosteum of 3-, 9-, and 18-month-old mice, which revealed age-related shifts in progenitor, neutrophil, and macrophage subpopulations. Aging reduced mesenchymal cell populations and impaired osteogenic potential, may contribute to periosteal homeostasis. Periosteal progenitor subsets exhibited distinct aging trajectories: Dpt⁺ fibrous-layer cells undergoing early senescence, while Postn⁺ progenitors showed osteogenic decline. Aging also shifted immune profiles, increasing inflammatory Cd38

The following analysis is generated by AI and may contain errors. It is separate from the authors’ abstract and is not peer review.

Research summary

Aging alters periosteal progenitor cell populations and immune microenvironment, impacting bone homeostasis and regeneration. The study addresses cellular and molecular mechanisms of aging, which are crucial for understanding the root causes of age-related decline in bone health.

Author of this paper? Send a correction.