Modifications of the Secretome of Mesenchymal Stromal Cells under Conditions of Stress-Induced Aging.

D N Kashirina, A Yu Ratushny, D K Matveeva, M I Ezdakova, L Kh Pastushkova, I M Larina, L B Buravkova

Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia. [email protected] of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia. [email protected]. · pubmed

Paper

Abstract

It is known that the senescence-associated secretory phenotype (SASP) can promote senescence of surrounding normal cells. However, SASP signaling during senescence of mesenchymal stromal cells (MSCs) has not yet been fully studied. We determined the pattern of secreted proteins specific to MSCs under stress-induced senescence. Using chromatography-mass spectrometry, proteins specific to the secretome of senescent or "young" cells were identified. The secretome of senescent cells contains proteins both associated with senescence (LOXL2, CCL2, PLAT, SERPINE2, etc.) and important for reducing the impact of these processes, in particular, proteins responsible for inhibition of oxidative stress (MIF, PRDX5, GSTM2), detoxification of methylglyoxal (GLO1), and suppression of inflammatory reactions (GAS6, GSTM2). The obtained results indicate the complex etiology of aging and the ambiguity of the function of SASP within the paracrine induction of aging of neighboring cells.

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Research summary

The paper identifies specific proteins in the secretome of mesenchymal stromal cells under stress-induced senescence. This research is relevant as it explores the mechanisms of cellular senescence and the role of the SASP, which are critical to understanding the aging process and potential interventions for age-related decline.

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