An Ad Libitum-Fed Diet That Matches the Beneficial Lifespan Effects of Caloric Restriction but Acts via Opposite Effects on the Energy-Splicing Axis.

Amanda E Brandon, Tamara Pulpitel, Carsten Schmitz-Peiffer, Lewin Small, Alistair M Senior, Sophie Stonehouse, Letisha Prescott, Alyssa Face, K Saiful Islam, Jenny E Gunton, Jacob George, David Raubenheimer, Gregory J Cooney, David G Le Couteur, Stephen J Simpson

Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia. · pubmed

Paper

Abstract

Caloric restriction (CR) with fasting extends lifespan but is difficult to maintain in humans. Here, we compared conventional CR with periods of fasting to an ad libitum-fed low-protein, high-carbohydrate (LPHC) diet diluted 25% with non-digestible fibre. Both approaches similarly enhanced longevity and metabolic health in mice relative to a control diet. Proteomic analysis of liver tissue revealed that CR increased proteins associated with energy and mitochondrial pathways. By contrast, the LPHC diet reduced these pathways but increased the abundance of proteins associated with RNA metabolism and spliceosome pathways. These results for LPHC support the "energy-splicing resilience" axis theory of ageing. Our results suggest that ad libitum-fed diets can be designed to replicate, and potentially enhance, the geroprotective benefits of CR, albeit via different mechanisms, potentially offering a more sustainable dietary approach to longevity extension.

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Research summary

The paper claims that an ad libitum-fed low-protein, high-carbohydrate diet can replicate the lifespan-extending effects of caloric restriction through different biological mechanisms. This research is relevant as it explores dietary interventions that may address the root causes of aging and longevity, offering potential alternatives to caloric restriction that could be more sustainable for human application.

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