MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution.

Ana Hernández de Sande, Tanja Turunen, Maria Bouvy-Liivrand, Tiit Örd, Senthil Palani, Mari Lahnalampi, Celia Tundidor-Centeno, Heidi Liljenbäck, Jenni Virta, Henri Niskanen, Buddika Jayasingha, Olli-Pekka Smålander, Lasse Sinkkonen, Lea Mikkola, Thomas Sauter, Anne Roivainen, Tapio Lönnberg, Minna U Kaikkonen, Merja Heinäniemi

School of Medicine, University of Eastern Finland, 70200, Kuopio, North-Savo, Finland. · pubmed

Paper

Abstract

Regulatory networks controlling aging and disease trajectories remain incompletely understood. MicroRNAs (miRNAs) are a class of regulatory non-coding RNAs that contribute to the regulation of tissue homeostasis by modulating the stability and abundance of their target mRNAs. MiRNA genes are transcribed similarly to protein-coding genes which has facilitated their annotation and quantification from bulk transcriptomes. Here, we show that droplet, spatial, and plate-based single-cell RNA-sequencing platforms can be used to decipher miRNA gene signatures at cellular resolution to reveal their expression dynamics in vivo.

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Research summary

The paper claims to reveal miRNA gene signatures and their expression dynamics in immune cell subpopulations during aging and atherosclerosis. This research is relevant as it explores the regulatory mechanisms of miRNAs in the context of aging and disease, potentially contributing to understanding the biological processes underlying aging and age-related diseases.

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