Protocol to assess the cytotoxicity of autologous human skin immune cells against senescent fibroblasts.

Tatsuya Hasegawa, Tomonori Oka, Shadmehr Demehri

Center for Cancer Immunology, Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Cutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Shiseido Global Innovation Center, Yokohama 220-0011, Japan. Electronic address: [email protected] for Cancer Immunology, Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Cutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Shiseido Global Innovation Center, Yokohama 220-0011, Japan. Electronic address: [email protected]. · pubmed

Paper

Abstract

Immune clearance of senescent cells contributes to their control in aging tissues; however, it remains unclear how immunity against senescent cells is regulated in humans. Here, we provide a protocol to measure the cytotoxicity of autologous human skin immune cells against senescent dermal fibroblasts. We describe the ex vivo co-culture experiments using normal and senescent fibroblasts together with autologous immune cells isolated from the human skin. This protocol is applicable to understanding the mechanism of immune surveillance and clearance of senescent cells in humans. For complete details on the use and execution of this protocol, please refer to Hasegawa et al.

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Research summary

The paper presents a protocol to measure the cytotoxicity of human skin immune cells against senescent fibroblasts. This research is relevant as it explores the immune mechanisms involved in the clearance of senescent cells, which is a key aspect of aging and longevity.

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