Fabrication and in-depth analysis of a novel-hybrid TPGS phytosome system for enhanced anti photoaging efficacy of grape seed extract.

Hanan Refai, Mona Elhabak, Doaa H Hassan, Omar S Ahmed, Esraa M Mohamed, Heba S Rateb, Nesrine S El Sayed, Mariam K Ahmed, Pierre Waffo-Téguo, Josep Valls, Nermeen Z Abuelezz

Department of Pharmaceutics, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, P.O. Box 77, 6(th) of October City, Giza, Egypt. · pubmed

Paper

Abstract

Photoaging is a widespread skin health concern caused by UV radiation. It significantly contributes to premature wrinkles and exacerbates further skin damage. This study evaluates the enhanced anti-aging efficacy of a nano phytosome incorporating grape seed extract (GSE) as an innovative, naturally driven antiaging formula, demonstrating putative skin permeability. LC-HRMS analysis and molecular docking revealed the potential of GSE components against photoaging targets. A GSE-nano phytosome formula with phosphatidyl choline (PC)/GSE ratio of 50:1 and 10 mg Vitamin E TPGS displayed optimal physicochemical properties, including particle size (195.1 ± 0.4 nm), polydispersity index (0.312 ± 0.015), zeta potential (25.6 ± 0.4 mV), and complexation efficiency (76.2 ± 4.5 %). FTIR analysis confirmed the formation of hybrid phytosomes through complexation with PC and TPGS. ex vivo confocal microscopy displayed enhanced skin permeability. Biochemical studies confirmed the superior antiaging effect of GSE-phytosome(GSE-nanogel) compared to crude GSE in UV-irradiated rats. GSE nanogel promoted anti-aging by suppressing c-Jun N-terminal kinase (JNK) and NF-κB, caspase-3, enhancing GSH-Px level and reducing malondialdehyde level. These effects suppressed elastase and collagenase activity, significantly improving wrinkles and skin redness. Our findings underscore the efficiency of formulating high molecular weight GSE phytoconstituents as phytosomes that mimic the lipophilic nature of cell membranes, improve skin permeability, and therapeutic efficacy.

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Research summary

The study claims that a novel GSE-nano phytosome system enhances anti-photoaging efficacy. The paper is relevant as it addresses a specific aspect of skin aging and proposes a formulation that may improve skin health, contributing to the broader understanding of aging mechanisms.

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