FluMag-SELEX derived specific individual aptamers can fluorescently label the ageing immanent protein-crosslinker glucosepane in diabetic mouse tissues.

Runliu Li, Bastian Draphoen, Mika Lindén, Karmveer Singh, Karin Scharffetter-Kochanek, Andreas Stürmer, Frank Rosenau, Ann-Kathrin Kissmann

Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany. [email protected] of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany. [email protected]. · pubmed

Paper

Abstract

Nowadays, aptamers have transitioned into valuable antibody alternatives. We present the first anti-glucosepane aptamer targeting a key glycation product linked to aging and diabetes. Glu3, with high specificity and affinity, enabled the first-ever direct, fluorescence-based histological staining of glucosepane in murine tissue, distinguishing diabetic samples from wild-type samples without secondary reagents.

The following analysis is generated by AI and may contain errors. It is separate from the authors’ abstract and is not peer review.

Research summary

The paper presents a novel anti-glucosepane aptamer that enables fluorescence-based histological staining of glucosepane in diabetic mouse tissues. This research is relevant as it targets glucosepane, a key glycation product associated with aging and diabetes, potentially addressing underlying mechanisms of age-related diseases.

Author of this paper? Send a correction.