Circadian clocks and periodic anticipated fasting prevent fasting-associated hepatic steatosis in calorie restriction.

Oghogho P Ebeigbe, Volha Mezhnina, Artem Astafev, Nikkhil Velingkaar, Jillian Kodger, Allan Poe, Jonathan Fritz, Kadaia Z Williams, Evelina Trokhimenko, Josefa-Marie B Rom, Yana Sandlers, Roman V Kondratov

Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University, Cleveland, OH 44115, USA; Department of Biological Geological and Environmental Sciences, Cleveland State University, Cleveland, OH 44115, USA.Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University, Cleveland, OH 44115, USA; Department of Biological Geological and Environmental Sciences, Cleveland State University, Cleveland, OH 44115, USA. · pubmed

Paper

Abstract

Calorie restriction (CR) improves health and longevity. CR induces a periodic fasting cycle in mammals; our study compares CR with unanticipated fasting (F), when the food is unexpectedly withheld. F induces hepatic steatosis, whereas CR reduces it; surprisingly, the difference is not due to hepatic β-oxidation. Liver transcriptome analysis identifies fatty acid transporters (Slc27a1 and Slc27a2), triglyceride (TAG) synthesis (Gpat4), and lipid storage (Plin2 and Cidec) genes to be upregulated only in F, in agreement with hepatic steatosis. The circadian clock and anticipated fasting contribute to preventing fasting-associated hepatic steatosis in CR. Mechanistically, the Slc27a1, Plin2, and Cidec genes are upregulated, and liver TAGs accumulate in circadian clock mutant mice on CR or if wild-type CR mice miss their anticipated meal. The results highlight the similarities and differences between F and CR, suggesting that circadian clock-dependent gating of transcriptional response to fasting controls lipid homeostasis and prevents hepatic steatosis.

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Research summary

The study claims that circadian clocks and anticipated fasting prevent fasting-associated hepatic steatosis in calorie restriction. This research is relevant as it explores mechanisms that may influence metabolic health and longevity through the regulation of lipid homeostasis, which is a key factor in aging and age-related diseases.

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