Impact of aging on gene expression in human oocytes: a comparative analysis of young and older patients.

Raoul Orvieto, Omri Nayshool, Louisa Cohen, Yuval Yung, Adva Aizer, Efrat Glick Saar, Dan Dominissini

Infertility and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center (Tel Hashomer), Ramat Gan, Israel. [email protected] and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center (Tel Hashomer), Ramat Gan, Israel. [email protected]. · pubmed

Paper

Abstract

Aging affects gene expression in pathways essential for energy metabolism, DNA repair, cell cycle regulation, and antioxidant defenses, directly affecting oocyte quality and viability. Single-cell RNA deep sequencing studies of aged versus young human MII oocytes revealed many differentially expressed genes. In addition, single human oocyte transcriptome analysis at both germinal vesicle (GV) and MII stages revealed distinct stage-dependent pathways impacted by aging, with a decrease in mitochondrial-related transcripts from GV to MII oocytes, and a much greater reduction in MII oocytes with advanced age.

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Research summary

Aging negatively impacts gene expression in human oocytes, affecting their quality and viability. The study addresses the molecular mechanisms underlying aging in oocytes, which is crucial for understanding reproductive aging and its implications for longevity.

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