Identification and molecular mechanisms of novel antioxidant peptides from Manila clam hydrolysate: Caenorhabditis elegans model, molecular simulations, and quantum chemistry.

Aijing Jia, Yucong Xu, Chaonan Sun, Hongwei Luan, Zhiteng Liu, Ying Bu, Wenhui Zhu, Xuepeng Li

College of Food Science and Engineering, Bohai University. Jinzhou, Liaoning 121013, China.College of Food Science and Engineering, Bohai University. Jinzhou, Liaoning 121013, China. · pubmed

Paper

Abstract

The objective of the study was to identify antioxidant peptides from Manila clam hydrolysates. Results demonstrated that the enzymatic hydrolysate of Manila clam (MW < 1 kDa) (RPPH-1) exhibited significant in vitro antioxidant activity and in vivo anti-aging effects. Specifically, the activities of glutathione peroxidase (GSH-Px) in the 1.5 mg/mL RPPH-1 dosage group increased by 1.44-fold compared with the control group. The lifespan of Caenorhabditis elegans was prolonged. Among the 19 identified peptides, FDCSQFKPEE (FE10) formed van der Waals and hydrophobic interactions with Keap1 via residues Leu557, Leu365, Ile559, Tyr334, and Phe577, indicating stability of the FE10-Keap1 complex. Quantum chemical analysis revealed that the active site of FE10 was Phe N

The following analysis is generated by AI and may contain errors. It is separate from the authors’ abstract and is not peer review.

Research summary

The study identifies antioxidant peptides from Manila clam hydrolysates that can prolong lifespan in Caenorhabditis elegans. The research is relevant as it explores potential mechanisms for lifespan extension through antioxidant activity, addressing aspects of aging.

Author of this paper? Send a correction.