Rejuvenation of Senescent Cells, In Vitro and In Vivo, by Low-Frequency Ultrasound.

Sanjay K Kureel, Rosario Maroto, Maisha Aniqua, Simon Powell, Ekta Singh, Felix Margadant, Brandon Blair, Blake B Rasmussen, Michael P Sheetz

Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA. · pubmed

Paper

Abstract

The presence of senescent cells causes age-related pathologies since their removal by genetic or pharmacological means, as well as possibly by exercise, improves outcomes in animal models. An alternative to depleting such cells would be to rejuvenate them to promote their return to a replicative state. Here we report that treatment of non-growing senescent cells with low-frequency ultrasound (LFU) rejuvenates the cells for growth. Notably, there are 15 characteristics of senescent cells that are reversed by LFU, including senescence-associated secretory phenotype (SASP) plus decreased cell and organelle motility. There is also inhibition of β-galactosidase, p21, and p16 expression, telomere length is increased, while nuclear 5mC, H3K9me3, γH2AX, nuclear p53, ROS, and mitoSox levels are all restored to normal levels. Mechanistically, LFU causes Ca

The following analysis is generated by AI and may contain errors. It is separate from the authors’ abstract and is not peer review.

Research summary

Low-frequency ultrasound rejuvenates senescent cells, reversing multiple characteristics of cellular aging. The paper addresses the rejuvenation of senescent cells, which is directly related to the underlying mechanisms of aging and has potential implications for longevity and age-related diseases.

Author of this paper? Send a correction.