Expression and characterization of colanic acid-degrading enzyme from Escherichia phage phi92 and analysis of its hydrolysate composition and structure.

Xiaotong Xu, Xiaoshuang Yan, Xuerong Jin, Juyi Li, Qiaoxia Hu, Dong-Hyun Ahn, Chao Zhong

Department of Food Science and Technology, Pukyong National University, Busan, Republic of Korea; Key Laboratory of Quantitative Synthetic Biology, Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.Department of Food Science and Technology, Pukyong National University, Busan, Republic of Korea; Key Laboratory of Quantitative Synthetic Biology, Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China. · pubmed

Paper

Abstract

Colanic acid (CA), an exopolysaccharide synthesized by bacteria, is known for its potential to extend the lifespan of nematodes. However, its high viscosity poses practical application challenges, which can be mitigated by employing colanic acid-degrading enzymes (CAEs). This study presents the expression and characterization of a novel CAE from Escherichia phage phi92, used for the production of CA oligosaccharides (CAOSs). Optimal CAE expression conditions were identified as induction at 20 °C for 24 h with 0.1 mM IPTG. The enzyme showed maximal activity at 55 °C and pH 6.0, with stability in the range of 4-50 °C and pH 4.0-7.0. Structural characterization of CAOSs was performed using GC-MS, LC-MS and NMR spectroscopy, revealing the composition of hexasaccharide and dodecasaccharide units, with CAE cleaving the β-1,4 glycosidic linkage between glucose and fucose. CAOSs were found to mitigate oxidative stress and inflammation induced by H

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Research summary

The study claims that colanic acid oligosaccharides can mitigate oxidative stress and inflammation. The research is relevant as it explores a potential mechanism (through CA oligosaccharides) that could influence longevity by addressing oxidative stress, a known factor in aging.

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