Integrating single-cell RNA and T cell/B cell receptor sequencing with mass cytometry reveals dynamic trajectories of human peripheral immune cells from birth to old age.

Yufei Wang, Ronghong Li, Renyang Tong, Taiwei Chen, Mingze Sun, Lingjie Luo, Zheng Li, Yifan Chen, Yichao Zhao, Chensheng Zhang, Lin Wei, Wei Lin, Haoyan Chen, Kun Qian, Alex F Chen, Junling Liu, Lei Chen, Bin Li, Feng Wang, Li Wang, Bing Su, Jun Pu

Department of Cardiology, Renji Hospital, School of Medicine, State Key Laboratory of Systems Medicine for Cancer, Shanghai Cancer Institute, Shanghai Jiao Tong University, Shanghai, China.Department of Cardiology, Renji Hospital, School of Medicine, State Key Laboratory of Systems Medicine for Cancer, Shanghai Cancer Institute, Shanghai Jiao Tong University, Shanghai, China. · pubmed

Paper

Abstract

A comprehensive understanding of the evolution of the immune landscape in humans across the entire lifespan at single-cell transcriptional and protein levels, during development, maturation and senescence is currently lacking. We recruited a total of 220 healthy volunteers from the Shanghai Pudong Cohort (NCT05206643), spanning 13 age groups from 0 to over 90 years, and profiled their peripheral immune cells through single-cell RNA-sequencing coupled with single T cell and B cell receptor sequencing, high-throughput mass cytometry, bulk RNA-sequencing and flow cytometry validation experiments. We revealed that T cells were the most strongly affected by age and experienced the most intensive rewiring in cell-cell interactions during specific age. Different T cell subsets displayed different aging patterns in both transcriptomes and immune repertoires; examples included GNLY

The following analysis is generated by AI and may contain errors. It is separate from the authors’ abstract and is not peer review.

Research summary

The paper claims to reveal dynamic trajectories of human peripheral immune cells across the lifespan, highlighting age-related changes in T cell subsets. This research is relevant as it investigates the evolution of the immune system throughout aging, which is crucial for understanding the biological processes underlying longevity and age-related diseases.

Author of this paper? Send a correction.