Identification of components that increase NAD+ levels in oxygen-glucose deprived HUVEC s from Schisandra chinensis (Turcz.) Baill. Based on spectrum-effect correlation analysis and target cell extraction.

Liwenyu Chen, Yi Yu, Min Lin, Ming Liang, Chen Yang, Xutian Kan, Xiaoliang Lin, Jin Qi

Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, PR China. · pubmed

Paper

Abstract

Schisandra chinensis (Turcz.) Baill, a traditional Chinese medicine with significant nourishing functions, has a regulatory effect on the cardiovascular system, digestive system, central nervous system, and endocrine and immune systems. It can protect the cardiovascular system, improve immunity, and has anti-oxidant and anti-aging properties. This study aimed to identify the S. chinensis components that increased NAD+ levels, by using spectrum-effect analysis and experimental validations. First, the quality of the S. chinensis extract was analyzed by HPLC-MS. The extract of S. chinensis increased the NAD+ levels of HUVECs during oxygen and glucose deprivation injury, and protected HUVEC s from injury from superoxide dismutase (SOD) and aging. The relationships of spectral effects were studied by partial least square regression. Coupled with target cell extraction, the material basis for increasing NAD+ levels in S. chinensis was obtained. The pharmacological activity of S. chinensis was verified at the cellular level. The related enzymes of the NAD+ synthesis and decomposition pathways were identified, showing that S. chinensis increased the level of NAD+ by increasing the activity of related enzymes in the synthesis pathways of NMNAT1, NMNAT2, NMNAT3, and NAMPT, but had no effect on the decomposition pathway. Finally, four constituents were confirmed, in vitro, to be the basis of S. chinensis-induced increases in the levels of NAD+. The EC

The following analysis is generated by AI and may contain errors. It is separate from the authors’ abstract and is not peer review.

Research summary

The paper investigates the effects of Schisandra chinensis on increasing NAD+ levels, which is relevant to longevity research as NAD+ is associated with cellular metabolism and aging processes. However, while the findings contribute to understanding the mechanisms behind NAD+ regulation, the study appears to be more of an incremental advance rather than a significant breakthrough in the field of longevity. The focus on specific components of a traditional medicine and their effects on cultured cells does not provide a comprehensive solution to the root causes of aging or lifespan extension.

Author of this paper? Send a correction.