Single-cell analysis of human peripheral blood reveals high immune response activity in successful ageing individuals.

Yu Wang, Yuxing Zhang, Ge Gong, Quanzhong Liu, Liangyu Li, Mingjiong Zhang, Shuping Shen, Ran Wang, Jianqing Wu, Wei Xu

Key Laboratory of Geriatrics of Jiangsu Province, Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; Department of Geriatrics, The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing 210031, China. · pubmed

Paper

Abstract

Beneficial remodeling of the immune system in successful ageing individuals (centenarians and supercentenarians) is critical for healthy ageing. However, mechanisms for dynamic regulation of immunity during ageing remain unclear. We use single-cell RNA sequencing (scRNA-seq) as an analytical strategy to study the dynamic regulation of immunity during aging and its molecular mechanisms at the single-cell level. We performed an integrative analysis of 87,215 peripheral blood mononuclear cells, from seven supercentenarians, three centenarians, and four elderly controls, generated by single-cell transcriptomics complemented with fluorescence-activated cell sorting. Animals experiments were also conducted to validate the makers of healthy aging found by our bioinformatic analysis and further explore the dynamic of immune changes during aging process. We found that CD8

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Research summary

The paper investigates the immune response in successful aging individuals, focusing on the dynamic regulation of immunity, which is pertinent to understanding the biological mechanisms of aging. While it provides solid research through the use of advanced techniques like single-cell RNA sequencing, the findings appear to be incremental rather than groundbreaking, limiting its overall impact on the field of longevity research.

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