Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis.

Jérémy Boulestreau, Marie Maumus, Giuliana Bertolino Minani, Christian Jorgensen, Danièle Noël

IRMB, University of Montpellier, INSERM, Montpellier, France.IRMB, University of Montpellier, INSERM, Montpellier, France. · pubmed

Paper

Abstract

Age is the most important risk factor for degenerative diseases such as osteoarthritis (OA). It is associated with the accumulation of senescent cells in joint tissues that contribute to the pathogenesis of OA, in particular through the release of senescence-associated secretory phenotype (SASP) factors. Mesenchymal stromal cells (MSCs) and their derived extracellular vesicles (EVs) are promising treatments for OA. However, the senoprotective effects of MSC-derived EVs in OA have been poorly investigated. Here, we used EVs from human adipose tissue-derived MSCs (ASC-EVs) in two models of inflammaging (IL1β)- and DNA damage (etoposide)-induced senescence in OA chondrocytes. We showed that the addition of ASC-EVs was effective in reducing senescence parameters, including the number of SA-β-Gal-positive cells, the accumulation of γH2AX foci in nuclei and the secretion of SASP factors. In addition, ASC-EVs demonstrated therapeutic efficacy when injected into a murine model of OA. Several markers of senescence, inflammation and oxidative stress were decreased shortly after injection likely explaining the therapeutic efficacy. In conclusion, ASC-EVs exert a senoprotective function both

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Research summary

The paper investigates the senoprotective effects of extracellular vesicles from mesenchymal stromal cells on senescent chondrocytes in osteoarthritis, which is a degenerative disease associated with aging. By addressing the accumulation of senescent cells and their role in the pathogenesis of OA, the research touches on mechanisms related to aging and potential interventions that could mitigate age-related cellular dysfunction. However, while the findings are solid and contribute to understanding the therapeutic potential of MSC-derived EVs, the impact is limited as it primarily focuses on a specific disease rather than broader mechanisms of aging or lifespan extension.

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