Rotating cell culture system-induced injectable self-assembled microtissues with epidermal stem cells for full-thickness skin repair.
Abstract
Epidermal stem cells (EpSCs) are crucial for wound healing and tissue regeneration, and traditional culture methods often lead to their inactivation. It is urgent to increase the yield of high quality EpSCs. In this study, primary EpSCs were isolated and cultured in a serum-free, feeder-free culture system. EpSCs are then expanded in a dynamic 3D environment using a rotating cell culture system (RCCS) with biodegradable porous microcarriers (MC). Over a period of 14 days, the cells self-assembled into microtissues with superior cell proliferation compared to 3D static culture. Immunofluorescence and qPCR analyses consistently showed that the stemness of the 3D microtissues was preserved, especially the COL17A1 associated with anti-aging was highly expressed in RCCS induced microtissues.
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Research summary
The paper addresses the cultivation of epidermal stem cells in a dynamic 3D environment, which is relevant to tissue regeneration and potentially to the mechanisms of aging and wound healing. However, while it presents solid research on improving the yield and quality of stem cells, it does not directly tackle the root causes of aging or lifespan extension. The findings are important for advancing the field of regenerative medicine but have limited implications for broader longevity research.
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