Determination of 3-hydroxyanthranilic acid in the sweat of healthy older adults.

Kanlaya Prapainop Katewongsa, Preeyanuch Manohong, Yaowapa Trangan, Rungrat Palakai, Sirinapa Mysook, Thitirat Mantim, Pairoj Saonuam, Piyawat Katewongsa

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand. · pubmed

Paper

Abstract

3-Hydroxyanthranillic acid (3HAA) is one of the key metabolites from the tryptophan (TRP) metabolism pathway and is associated with aging, age-associated diseases, and healthy lifespan extension. This study aims to detect 3HAA in the sweat of healthy older adults using simple, high-performance liquid chromatography (HPLC) method. Chromatographic separation using 20 mmol/L sodium acetate, 3 mmol/L zinc acetate, and 7% (v/v) acetonitrile as mobile phase is possible to simultaneous detect 3HAA, KYN, and TRP with UV and fluorescence detection, respectively, under 6 min. This method demonstrated excellent linearity with coefficient of determination (r

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Research summary

The paper investigates the detection of 3-hydroxyanthranilic acid, a metabolite linked to aging and age-associated diseases, in the sweat of healthy older adults. While it touches on a metabolite associated with aging, the study primarily focuses on a methodological approach rather than addressing the root causes of aging or lifespan extension directly. Thus, it contributes solid research but has limited impact on advancing the field of longevity research.

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